Phenotypic Detection of AmpC \(\beta\)-lactamase Producing Escherichia coli among Patients in Hospital Wards
نویسندگان
چکیده
Background and Objectives: AmpC β-lactamase producing Escherichia coli has emerged in hospital environments as an important health issue that is of a global concern drug-resistant pathogenic bacteria evolved into strains are resistant to many classes antibiotics. However, awareness the prevalence microorganisms such E. could be very valuable for achieving more accurate epidemiological results, well controlling their spread different wards.
 Methodology: A total four hundred (400) clinical samples comprising two seventy-nine (279) urine one Twenty-one (121) wound were collected from patients ward at AE-FUTHA. The analyze using standard microbiological culture identification coli. Detection phenotypic β-lactamases production was performed Cefoxitin-Cloxacillin Double-Disk Synergy Test (CC-DDST). Antibiotic susceptibility studies determined Kirby–Bauer disk diffusion method results construed Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints.
 Results: Phenotypic accounted overall rate 94.1%. frequency 57(93.4%) high occurrence Surgical 15(100%) followed by medical 24(88.9%) but also 38(95.0%) consisting 9(100 %) children 10(100%) Orthopedic 13(86.7%) with least rate. exhibited percentage resistance within range 50-100% against ceftriaxone, ceftazidime, cefepime, azetronam, Trimethoprim-Sulfamethoxazole Ticarcillin-clavulanic acid susceptible ofloxacin 70.0%, Imipenem 83.3% amikacin 100%.
 Conclusion: findings this study proof among admitted wards there’s urgent need managing development MDR genotype strain. Moreover, prevent other improve effectiveness antibiotics, it suggested establish precise schedule antibiotic use each region based on pattern.
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ژورنال
عنوان ژورنال: Journal of advances in microbiology
سال: 2023
ISSN: ['2456-7116']
DOI: https://doi.org/10.9734/jamb/2023/v23i1702